Immunofluorescence (IF) detection with an antibody against the central part of separase (amino acids 1200–1300) in bipolar mitoses from WiT49, HEK293, and control cell lines without MM (fibroblasts and CHP212 neuroblastoma cells) accordingly resulted in fluorescence confined to the spindle poles at metaphase and early anaphase (Figure 3B and C). Here, ESPL1 is linked to Miyoshi myopathy.