Daily subcutaneous (SC) treatment of 12 weeks old obese female db/db mice with 20 mg/kg of NT4 (n = 8 per group) for 30 days also led to a sustained 50–80% decrease in daily food intake (Fig. 1e), and a linearly time-dependent, 40% decrease in body weight (Fig. 1f), confirming that activation of TrkB alone causes anorexia and weight loss in rodents independent of the leptin signal [9]. This evidence concerns the gene NTF4 and Anorexia.