PRNP and scrapie: Verification of all the 12 differentially expressed genes was performed by real-time RT-PCR using total RNA isolated from whole ileal PP of 14 lambs; eight scrapie-infected lambs killed eight months after oral exposure with susceptible PrP genotypes (4 PrPARQ/VRQ and 4 PrPVRQ/VRQ) were compared with six controls of the same age and PrP genotype (3 PrPARQ/VRQ and 3 PrPVRQ/VRQ).