VIM and ductal breast carcinoma in situ: However, multicolour confocal imaging also reveals that expression of ER in the normal breast is associated with cells expressing high levels of Ck 8/18; that there is an upregulation of vimentin expression in the Ck 8/18 negative myoepithelial cells in the columnar cell change [see also Additional file 1(4)]; and that scattered vimentin-positive, Ck 8/18 negative cells admixed with neoplastic cells can be found in high grade DCIS.