TNF and breast cancer: As shown in Fig. 1B, migration of MDA-MB-231 cells cross HUVEC monolayers was significantly blocked by anti-E-selectin antibody, but not by inhibitory antibodies against LFA-1 or VLA-4 (Fig. 1B), suggesting that metastasis of breast cancer cells across TNF-α pre-activated HUVEC monolayers is specifically dependent on HUVEC E-selectin.