RPE65 and central nervous system cancer: To further characterize cell-specificity of the HRSE-6xHRE-Rpe65 promoter construct, we transiently transfected several additional nontarget cell lines, including HT22, HEK-293, C6 Glioma, HepG2, and A-172, exposing them to hypoxia for 40 h before analysis by DLA.