Secondly, breast cancers which have metastasised to the regional lymphatics show higher expression compared to those which have not, although the individual differences for all five markers were not statistically significant The VEGF-C:VEGF-D ratio ratio however was significantly higher in the node node +ve group which concurs with previous studies [21,22] which link this relationship to poor prognosis. This evidence concerns the gene VEGFC and breast carcinoma.