The combination of techniques led to the discovery of validated biomarkers for glioma vessels, respectively, fibronectin precursor with SwissProtTM accession code P02751, collagen-binding protein 2, also named colligin-2 with SwissProtTM accession code P50454, and one candidate marker acidic calponin-3 with SwissProtTM accession code Q15417. The gene discussed is FN1; the disease is glioma.