SFRP5 and breast carcinoma: Subsequent immunofluorescence analysis of the intracellular distribution of β-catenin in seven breast cancer cell lines (MCF-7, MDA-MB-231, MDA-MB-435S, MDA-MB-436, MDA-MB-468, T-47D and SK-BR-3) revealed nuclear accumulation of β-catenin in four cell lines (MCF-7, MDA-MB-231, T-47D and SK-BR-3) in which SFRP1, SFRP2 and SFRP5 were silenced (Supplementary Figure 1).