Western blot analysis showed that the protein level of the inactivated form of caspase-3 (35 kDa) was decreased, while the activated form of caspase-3 (17 kDa) appeared, and the caspase substrate PARP was diminished in the Ad-SOX2-infected OUMS37 cells at 48 and 72 h after infection compared with the control Ad-GFP-infected and parental cells (Figure 4D). This evidence concerns the gene SOX2 and infection.