After infection with YS001 or YS005 for 1 h at 37°C in the cell culture medium without supplementation of any antibiotic followed by subsequent incubation for 1 h in fresh medium supplemented with GEN to kill extracellular bacteria, the infected monolayers were further incubated with fresh culture medium supplemented with each antibiotic (AMP, AZM, CLR, CFPN, or LVX; 1 μg/ml) or not supplemented for 2 h at 37°C. Here, DCLK3 is linked to infection.