The current investigation demonstrates the potential of the designed templates OAcSGP+CD10+CD19+ or OAcSGP+CD34+CD19+ or OAcSGP+CD7+CD3+ for successful diagnosis and evaluation of the disease status during the two-year chemotherapy, suggesting its application in MRD detection and prediction of impending relapse in ALL both in PB and BM. The gene discussed is CD34; the disease is acute lymphoblastic leukemia.