The expression of seven of the twelve genes was significantly different when comparing the non muscle invasive tumors without concomitant CIS to both the tumor with concomitant CIS and to the muscle invasive tumors (t-test, ATF2, p-value = 1.2E-4; CREBBP, p-value = 1.2E-2; MEF2A, p-value = 1.7E-4; MEF2C, p-value = 1.7E-3; ATF1, p-value = 2.6E-2; STAT1, p-value = 1.3E-4; STAT3, p-value = 1.2E-2). This evidence concerns the gene MEF2C and in situ carcinoma.