We determined the feasibility of this RMEAM strategy by assessing the methylation density of CpG islands located in the promoter regions of MLH1, TERT and MGMT (Fig. 2A), and it has been proved that the mathylation of MLH1, TERT and MGMT are associated with carcinogenesis of colorectal tumor. The gene discussed is MLH1; the disease is colorectal neoplasm.