we screened various human and murine tumor cell lines from different tissues, including human cell line Hela (cervical cancer), A549 (lung carcinoma), Hep2 (laryngeal carcinoma), HepG2 (hepatoma), HO8910 (ovarian epithelial carcinoma), and murine cell line B16 (melanoma), RM1(prostate cancer), LLC (lung cancer), MCA38 (colon cancer), Hepa1-6 (hepatocellular carcinoma) for the expression of TLR3 by RT-PCR (Fig 1A) and FACS analysis (Fig 1B, C). The gene discussed is TLR3; the disease is hepatocellular carcinoma.