To ascertain whether CD47/IAP is required for mechanical signalling at different frequencies of stimulation, CD47/IAP-null cells (OV10, human carcinoma cell lines, and mouse CD47/IAP-/- lung fibroblasts) and cells transfected with CD47/IAP type 2 DNA or vector controls were used and the electrophysiological membrane response was assessed. This evidence concerns the gene CD47 and carcinoma.