To address the functional impact of TGF-β1-induced uPA production on the progression of prostate cancer in the microenvironment of SV, it would be absolutely necessary to perform further experiments characterising changes in malignant phenotype of PC3 cells both in vitro and in vivo before and after the inactivation of TGF-β1 and/or uPA. This evidence concerns the gene PLAU and prostate carcinoma.