We evaluated liver fibrosis employing the following methods: i) microscopic morphology; ii) semi-quantitative METAVIR clinical grading system; iii) collagen type 1 immunofluorescence; iv) quantitative Sirius red collagen-binding assay; v) quantitative hydroxyproline collagen content; vi) RT-PCR for collagen type 1 mRNA; vii) RT-PCR for α-smooth muscle actin (α-SMA) mRNA (present in activated HSC); and viii) RT-PCR for transforming growth factor (TGF-β) mRNA (a pro-fibrotic cytokine) [4], [7], [8]. Here, ACTA1 is linked to Hepatic fibrosis.