It has also been reported that high MVD is an independent prognostic factor in node-negative IHCC (Shirabe et al, 2004) and is associated with VEGF expression in IHCC (Tang et al, 2006), although no study has clarified the involvement of angiogenesis in the process of metastasis in cholangiocarcinoma. The gene discussed is VEGFA; the disease is cholangiocarcinoma.