We used enzyme-linked immuno-sorbent assay (ELISA) to determine IL-8 protein levels in culture supernatants of A549 and NCI-H292 cells at 24 h after infection with either the parental strain AA100jm or dotO mutant strain and either the parental strain Corby or flaA mutant strain at an MOI of 100. Here, CXCL8 is linked to infection.