Three SP1 PWMs, V$SP1_Q2_01, V$SP1_Q4_01 and V$SP1_Q6, were significant in the previous analyses at the binding site and gene levels, so we utilized these matrices to search for other TFs that may work in conjunction with an SP1-like factor to regulate intestinal genes during iron-deficiency. The gene discussed is SP1; the disease is Iron deficiency anemia.