The methylated DNAs were detected with the following frequency: 65.6% (21 of 32) in p16, 3.1% (1 of 32) in MGMT, 31.3% (10 of 32) in GSTP1, 87.5% (28 of 32) in APC, 0% (0 of 32) in DAPK, 56.3% (18 of 32) in RIZ1, 56.3% (18 of 32) in SFRP1, 53.1% (17 of 32) in SFRP2, 28.1% (9 of 32) in SFRP5, 46.9% (15 of 32) in RUNX3, and 46.9% (15 of 32) in SOCS1. There were no significant statistical differences between cases of multiple HCC and single HCC. The gene discussed is SOCS1; the disease is hepatocellular carcinoma.