Thirteen of the analyzed loci showed statistically significant differences in DNA methylation when AD samples were compared to all NTL samples: OPCML, CDX2, HOXA1, CDKN2A EX2, SFRP1, CDH13, TWIST1, LZTS1, RASSF1, SFRP4 and 5, ESR1, and CDH1. Here, SFRP4 is linked to Alzheimer disease.