Additional nullizygosity for p16Ink4a increases the rate of meningioma development and meningothelial proliferation in adCre;Nf2flox2/flox2 mice. To investigate the potential synergy of Nf2 inactivation and homozygous p16Ink4a mutation in meningioma development, a cohort of 32 Nf2flox2/flox2;Ink4a*/* mice were injected with adCre and compared with 80 adCre‐injected Nf2flox2/flox2. This evidence concerns the gene CDKN2A and meningioma.