For genes related to breast cancer estrogen signaling, GSEA analysis showed a progressive down-regulation of keratin 18 and NME1 from luminal A to basal tumors, which also correlated with ER(+)ve MDA-MB-435 cells; low expression of these genes correlates to poor prognosis and metastasis [21,22]. The gene discussed is NME1; the disease is breast cancer.