To examine the regulation of transcriptional activity of Runx2 by GSK-3β, a luciferase reporter gene construct containing a 1,050 bp osteocalcin gene fragment (1,050 OC-Luc) including the Runx2 binding sites was transfected into human hepatoma HuH-7 cells. The gene discussed is BGLAP; the disease is hepatocellular carcinoma.