Intracellular IFNγ staining of Thy1.1+ DUC18 T cells was conducted at days 2 and 6 post-transfer into CMS5 tumor bearing mice; no exogenous target cells were given i.t. or i.v. Similar to the previous study [43], tumor-infiltrating DUC18 CTL, but not those trafficking through dLNs or spleens, were found to be producing IFNγ when examined ex vivo (Figure 4D). Here, IFNG is linked to neoplasm.