We validated the expression of E2F1, RUNX3, EGR3 and TBPIP in leiomyomas [17], keloids, incisional scars and peritoneal adhesions showing a good correlation with microarray data Since activation of these signal transduction pathways and transcription factors regulate the expression of large number of genes with diverse functional activities their altered expression in these tissues could have a considerably more important role in tissue fibrosis than previously considered. The gene discussed is E2F1; the disease is leiomyoma.