CENPA and breast cancer: To better resolve the kinetics of nuclease digestion of CenH3 chromatin, we used diluted MNase across the same time course (1, 2, 3 min), wherein BC samples progress from 185 × 3, 185 × 2, 185 × 1 down to the core protection of 150 bp (Figure 4B, lanes 2–4 and red arrow on the densitometric scan below).