To obtain insight into the mechanisms underlying the reduced mortality of TLR2 KO mice during experimental melioidosis, we infected WT, TLR2 KO, and TLR4 KO mice with B. pseudomallei and sacrificed the mice after 24, 48, and 72 h (i.e., directly before the first predicted death in WT mice) to determine bacterial loads in lungs (the primary site of the infection), blood, and spleen (to evaluate extent of bacterial dissemination) (Figure 4). Here, TLR4 is linked to infection.