Figure 6E shows that the SERCA3 moderately to strongly labeled ER in the RIS and CIS (white arrows), closely apposed to the cone juxtanuclear mitochondria (white arrowheads), and in the OPL. Thus, the SERCA3 labeling pattern in the outer (distal) retina was similar to that of calreticulin. Double labeling experiments with the pan-SERCA3 and VGluT1 revealed that they completely and strongly colocalized in both rod spherules and cone pedicles (Figure 6F: purple pixels). The gene discussed is ATP2A3; the disease is in situ carcinoma.