We aimed (1) to resequence the entire human PNMT gene in hypertensive and normotensive individuals of European origin in order to uncover the fine-scale sequence variation and to identify novel hypertension-susceptibility polymorphisms; (2) to apply population genetics statistics and in silico methods to trace the evolutionary pressure on human PNMT and to explore a gene regulatory potential of PNMT first intron reflecting the effect of purifying selection. The gene discussed is PNMT; the disease is hypertensive disorder.