To further investigate whether the epigenetic events could contribute to the down-regulation of SFRP1, both the demethylation agent DAC and the histone deacetylase inhibitor TSA were employed to treat some HCC cell lines, such as Bel7404, QGY7701, MHCC-H cells, without the expression of endogenetic SFRP1 (Fig. 1E). Here, SFRP1 is linked to hepatocellular carcinoma.