SLC25A16 and melanoma: Figure 3B shows expression of VEGF165b in three melanoma samples, whereas VEGF165 was clearly seen in only one sample (melanoma number 2). It was therefore clear that VEGF165b was expressed in sections of human melanoma. However, these sections contained three different types of tissue, and mRNA was extracted from all three – normal skin, HGP melanoma and VGP melanoma. To localise the expression of the isoforms, we performed immunohistochemistry using a VEGFxxxb-specific antibody.