It was found, that calgranulin B (expressed by macrophages and by epithelial cells in acutely inflamed tissues) and a unique restrictive fragment of Insulin-like Growth Factor Binding Protein 1 (IGFBP-1) – synthesized by both fetal membranes and maternal decidua, were differentially expressed in the amniotic fluid and sera during intra-amniotic infection prior to the appearance of clinical symptoms. The gene discussed is IGFBP1; the disease is chorioamnionitis.