Our results demonstrate that an important difference in integrin and MMP2/9 expression exists between ovarian cancer cells grown as monolayers versus those grown as spheroids and that function blocking monoclonal antibodies against α2 and β1 integrin subunits and α2β1 integrin can block the disaggregation and MMP2/9 activation of ovarian carcinoma spheroids. The gene discussed is MMP2; the disease is ovarian carcinoma.