In this study, we analyzed the effect of TNF-α on proliferation and survival of human glioma cells and investigated the role of CDKIs-p21cip/waf1 and p27kip1 in context with p53 and activation of NF-κB and Akt pathways using monolayer cultures and MCS as in vitro models. The gene discussed is CDKN1B; the disease is glioma.