CD4 and infection: We accordingly assessed whether sorted CD4+CD25+ MLNC from infected animals were able to suppress the proliferation of CFSE-loaded naïve, CD4+CD25– MLNC, responding to Con A. We observed that, on a per-cell basis, CD4+CD25+ MLNC, taken 28 day post-infection, were substantially more suppressive than cells with similar phenotype from naïve animals (Fig. 4A, B).