Quantitation using image analysis software showed that the basal third of the epithelium (the crypts) stained significantly more intensely for phospho-Akt in non-dysplastic Barrett's oesophagus compared to squamous epithelium, and both HGD and adenocarcinoma stained significantly more intensely than non-dysplastic Barrett's (fig 2a). The gene discussed is AKT1; the disease is Barrett esophagus.