TNF and adenocarcinoma: To analyze the apoptosis network we considered a previously published protein signaling dataset known as the Cytokine compendium [6], for which quantitative western blotting, high-throughput protein kinase assays and protein microarrays were used to investigate the combinatorial effect of tumor necrosis factor (TNF), epidermal growth factor (EGF) and insulin on apoptosis of human adenocarcinoma cells.