For the purpose of assessing whether the increased level of TXNIP RNA determined by prolonged conditions of hyperglycemia (20 mM, chronic conditions) were correlated with correspondent elevated levels of TXNIP protein, we analyzed the expression of the protein in MDA-MB-231 cells grown "chronically" and stably in 20 mM glucose tissue culture media by Western blot. This evidence concerns the gene TXNIP and Hyperglycemia.