TXNIP and Hyperglycemia: In order to assess whether hyperglycemia exerted any action in regulating ROS as an indicator of oxidative stress in response to 20 mM glucose (hyperglycemia)-induced elevation of TXNIP RNA level, we measured the ROS level as detected by CM-H2DCFDA and expressed as percentage of mean fluorescence by flow cytometry (Figure 4B, inlet) in relation to the level of TXNIP RNA.