GSTM1 and hemoglobin measurement: In high-endemic areas of HBV infection, a major route of transmission is perinatal and timing of the birth dose appeared to be the most important factor in achieving protective efficacy and to prevent perinatal HBV transmission.22 In this study, the coverage of timely given birth HB vaccine was significantly lower in rural areas and Province centers compared to Metropolitan cities, whereas in contrast the late administration of birth HB was more likely to be common.