Because syringomyelia, a persistent patency of the central canal within the spinal cord, is often associated with Chiari type I malformation [26] and was observed in DGAP205-1s but not previously noted in Nfia−/− mice [18], we first examined Nfia expression in the developing spinal cord (Figure 3A–3D). Here, NFIA is linked to Chiari malformation type I.