In the AOM/DSS group, tensin-like SH2 domain containing 1 (Tens1), ring finger protein 25 (Rnf25), carbonic anhydrase 8 (Car8), solute carrier family 13 (sodium/sulphate symporters), member 1 (Slc13a1), regulator of G-protein signaling 17 (Rgs17), prolactin receptor (Prlr), and complement receptor related protein (Crry) were significantly down-regulated at wk 5. The gene discussed is RNF25; the disease is infectious otitis media.