Helicobacter felis (H. felis)-infection of InsGas mice enhanced transformation of the gastric mucosa (Wang et al, 2000), and so we aimed to compare Shh pathway activation between pre-metaplastic and pseudopyloric metaplastic lesions in the non-infected and H. felis-infected InsGas models, respectively, focussing on Shh target genes, Patched-1 (Ptch-1) and glioma-associated oncogene (Gli)-1 and a factor, which regulates the pathway but which is not a transcriptional target, Gli-3. This evidence concerns the gene SHH and infection.