PBMCs from nine patients with melanoma and nine healthy controls were stimulated with 1,000 IU/ml IFN-α, IFN-β, or IFN-γ (or left unstimulated), and phosphorylation of STAT1 at tyrosine 701 was measured using Phosflow (BD Biosciences). The gene discussed is IFNA1; the disease is melanoma.