IFI44 and melanoma: Real-time qPCR was performed for four ISGs shown to be significantly down-regulated in the microarray data: STAT1, IFIT1, IFI44, and MX2. The expression level of these genes in IFN-α~-stimulated cells was lower in the melanoma group compared to the healthy group, although the difference in expression was not statistically significant for three of the four genes (Figure 4A).