VCAN and central nervous system cancer: To evaluate the profiles of TGF-β2 and versican in two human glioma cell lines (U87, A172) and in five primary cell cultures (HTZ-324, HTZ-349, HTZ-417, HTZ-419, HTZ-421), expression levels of TGF-β2 and versican isoforms were detected by RT–PCR at mRNA level using β-actin as control gene (Figure 1A).