MLANA and neoplasm: Besides, tumor-derived exosomes loaded onto DCs have been shown to trigger MART-1 melanoma Ag cross-presentation to specific CTLs [38] Although we used washed Apo-Nec cells resuspended in fresh AIM-V medium in all experiments and a differential ultracentrifugation of culture supernatants is required to obtain tumor-derived exosomes, we cannot exclude the contribution of exosomes that might be released by Apo-Nec cells during the 48 hs co-culture with DCs.