Controls for this experiment were performed, showing specific HLA-A*0201-restricted response using either DCs loaded with the corresponding peptides or CTL stimulation with live HLA-A*0201 positive gp100+ MelanA/MART-1+ melanoma cells (Mel-XY3) (Figure 6A and 6B) but lack of response using live MEL-XY2 cell line or with MelanA/MART-1 peptide for G154 clone or gp100 peptide for M27 clone. This evidence concerns the gene PMEL and melanoma.