ESR1 and neoplasm: In order to validate these mutant TP53-associated genes, we then assessed by quantitative RT-PCR the levels of expression of the first ten genes with the largest fold difference (Figure 2B; Table S7), as well as ESR1. Most of the 12 gene transcripts tested showed significant differences between TP53-mutant or wild-type tumors, not only in the tumor set used to select those genes, but also in the rest of the samples, the ESR1pos subgroup, and the complete population (Dataset S1; Figure 3).