HPN and metastatic malignant neoplasm in the lymph nodes: Expression of hepsin was highest on average in Gleason pattern 3 cells (5.6 ± 2.9 hepsin/beta-actin ratio), and was observed to decrease in high grade, Gleason pattern 5 and lymph node metastasis, samples (2.6 ± 1.5) to a level intermediate between benign (0.7 ± 0.4) and low grade, Gleason pattern 3, cells (Figures 5D–5F).